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| Registros recuperados : 142 | |
| 5. |  | VENTURI, S. S.; FONSECA, J. F. da; RIBEIRO, A. C. S.; ROGERIO, M. C. P.; OLIVEIRA, J.; ARAÚJO, A. R.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z. Energy supplementation in Santa Inês sheep subjected to estrus induction treatment. Semina: Ciências Agrárias, Londrina, v. 37, n. 1, p. 145-154, jan./fev. 2016.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 6. | | MAIA, A. L. R. S.; SILVA, M. R.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; FARIA, L. S.; CÔRTES, L. R.; FACO, O.; FONSECA, J. F. da. Epidemiological survey and risk factors associated with hydrometra in dairy goat herds. Small Ruminant Research, v. 178, p. 79-84, Sept. 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 7. | | ANDRADE, A. B. P. de; MORAIS, M. C. C.; RANGELM P. S. C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Effect of eCG in a short-term synchronization treatment on ovarian status, estrus synchrony, and ovulation in dairy goats managed under tropical conditions. Tropical Animal Health and Production, v. 53, n. 246, 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | TEIXEIRA, T. A.; FONSECA, J. F. da; SOUZA-FABJAN, J. M. G. de; CARVALHEIRA, L. de R.; FERNANDES, D. A. de M.; BRANDÃO, F. Z. Efficiency of different hormonal treatments for estrus synchronization in tropical Santa Inês sheep. Tropical Animal Health and Production, Heidelberg, v. 48, n. 3, p. 545-551, Mar. 2016.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | MORAIS, M. C. C.; ARRAIS, A. M.; SILVA, N. C.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Association of controlled light program, cloprostenol and gonadotropins for synchronous estrus induction and fixed time artificial insemination in dairy goats during the non-breeding season: preliminary results. Animal Reproduction, v. 17, n. 3, 2020. Edição dos Abstracts of the 34nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE), 2020 (online).| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | FONSECA, J. F. da; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; BRANDÃO, F. Z.; VIANA, J. H. M. Freezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 70, n. 5, p. 1489-1496, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | MAIA, A. L. R. e S.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; BALARO, M. F. A.; BRANDÃO, F. Z.; FONSECA, J. F. da. Distúrbios reprodutivos em cabras leiteiras e impactos potenciais nos sistemas de produção. Revista Acadêmica; Ciência Animal, Curitiba, v. 15, p. 77-89, 2017. Suplemento 2. Edição dos trabalhos apresentados no XI Congresso Brasileiro de Buiatria, Foz do Iguaçu, PR, 11 a 14 set. 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | PINHEIRO, J. B. S.; SOUZA-FABJAN, J. M. G.; CORREIA, L. F. L.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Differences between 5- and 6-day progestogen-based oestrus induction protocol in Saanen and Toggenburg goats. Reproduction, Fertility, and Development, v. 33, n. 2, p. 166, 2021. Edição dos Proceedings of the 47th Annual Conference of the International Embryo Technology (IETS) Virtual Meeting, January 18-21, 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | MAIA, A. L. R. S.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; ARAÚJO, M. C. C.; SIQUEIRA, L. G. B.; FACO, O.; FONSECA, J. F. da. Hydrosalpinx in dairy goats: occurrence, ultrasound diagnosis, macro- and microscopic characterization. Small Ruminant Research, v. 160, p. 5-11, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 16. | | FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G.; PRATES, J. F.; TREVIZAN, J. T.; BRANDÃO, F. Z.; FONSECA, J. F. da. Goat incubator: the doe as a life incubator of bovine oocytes - first step. Animal Reprodroduction, v. 14, n. 3, p. 738, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | MAIA, A. L. R. e S.; SOUZA-FABJAN, J. M. G. de; RANGEL, P. S. C.; CÔRTES, L. R.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Estrus induction in the non-breeding season is not associated with hydrometra in dairy goats. Research, Society and Development, v. 10, n. 11, e21101119162, 2021.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | CARVALHO-DE-PAULA, C. J.; SOUZA-FABJAN, J. M. G.; GONÇALVES, J. D.; DIAS, J. H.; SOUZA, G. N. de; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Effect of a 12-h increment in the short-term treatment regimen on ovarian status, estrus synchrony, and pregnancy rate in artificially inseminated dairy goats. Animal Reproduction Science, v. 221, e106571, 2020.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 20. | | MARTINS, B. B.; SOUZA-FABJAN, J. M. G.; DIÓGENES, Y. P.; OLIVEIRA, T. de A.; VIANA, J. H. M.; FONSECA, J. F. da. Cenário histórico da transferência de embriões de caprinos no Brasil: relatos técnicos científicos. Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 556, abr./jun. 2019. Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 142 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; Ribrio Ivan Tavares Pereira Batista; Juliane Teramachi Trevizan; Joanna Maria Gonçalves Souza-Fabjan; Felipe Zandonadi Brandão; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019. |
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DOI: |
10.5433/1679-0359.2019v40n6Supl3p3789 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation. MenosAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that trans... Mostrar Tudo |
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Palavras-Chave: |
Bovine oocytes; COCs; Ex-situ maturation; In vitro maturation; Nonsurgical embryo transfer technique. |
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Thesaurus NAL: |
Goats. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208736/1/Goat-incubator-can-bovine-oocytes.pdf
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Marc: |
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001 2118709
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008 2019 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2019v40n6Supl3p3789$2DOI
100 1 $aFONSECA, J. F. da
245 $aGoat incubator$bcan bovine oocytes be matured in the uterine horn of a goat?$h[electronic resource]
260 $c2019
520 $aAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation.
650 $aGoats
653 $aBovine oocytes
653 $aCOCs
653 $aEx-situ maturation
653 $aIn vitro maturation
653 $aNonsurgical embryo transfer technique
700 1 $aBATISTA, R. I. T. P.
700 1 $aTREVIZAN, J. T.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aBRANDÃO, F. Z.
700 1 $aCAMARGO, L. S. de A.
773 $tSemina: Ciências Agrárias, Londrina$gv. 40, n. 6, supp. 3, p. 3789-3796, 2019.
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Embrapa Gado de Leite (CNPGL) |
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